2024

62. Bridging structural biology and clinical research through in-tissue cryo-electron tomography

Kathryn Kixmoeller, Benjamin C Creekmore, Edward B Lee, Yi-Wei Chang✉

EMBO J (2024)

61. Toxoplasma gondii rhoptry discharge factor 3 is essential for invasion and microtubule-associated vesicle biogenesis

Rouaa Ben Chaabene, Matthew Martinez, Alessandro Bonavoglia, Bohumil Maco, Yi-Wei Chang, Gaëlle Lentini✉, Dominique Soldati-Favre✉

PLOS Biology (2024)

60. An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells

Christina M. Rothenberger, Manda Yu, Hey-Min Kim, Yee-Wai Cheung, Yi-Wei Chang, Mary Ellen Davey✉

Current Research in Microbial Sciences (2024)

59. The first Cryptosporidium meeting: a concerted effort to fight cryptosporidiosis

Wes van Voorhis, Joyce Siwila, Jessica C. Kissinger, Natalia Bayona Vásquez, Guy Robinson, Rodrigo Baptista, Asis Khan, Amandine Guérin✉, Yi-Wei Chang, Zannatun Noor, N. Bishara Marzook, Sumiti Vinayak, Sam Arnold, Chelsea Marie✉, Robert K.M. Choy, Mattie C. Pawlowic, Rajiv S. Jumani

Trends in Parasitology (2024)

58. Centromeric chromatin clearings demarcate the site of kinetochore formation

Kathryn Kixmoeller, Yi-Wei Chang✉, Ben E Black✉

bioRxiv (2024)

57. Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling

Benjamin C Creekmore*, Kathryn Kixmoeller*, Ben E Black, Edward B Lee✉, Yi-Wei Chang✉

Nature Communications (2024)

56. Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins

Nicolas Dos Santos Pacheco*, Albert Tell i Puig*, Amandine Guérin*, Matthew Martinez, Bohumil Maco, Nicolò Tosetti, Estefanía Delgado-Betancourt, Matteo Lunghi, Boris Striepen, Yi-Wei Chang✉, Dominique Soldati-Favre✉

Nature Communications (2024)

55. Combining per-particle cryo-ET and cryo-EM single particle analysis to elucidate heterogeneous DNA-protein organization

Leon Palao III, Kenji Murakami✉, Yi-Wei Chang✉

Current Opinion in Structural Biology (2024)

2023

54. Visualizing the membrane disruption action of antimicrobial peptides by cryo-electron tomography

Eric H.-L. Chen*, Chun-Hsiung Wang*, Yi-Ting Liao*, Feng-Yueh Chan, Yui Kanaoka, Takayuki Uchihashi, Koichi Kato, Longsheng Lai, Yi-Wei Chang, Meng-Chiao Ho✉, Rita P.-Y. Chen✉

Nature Communications (2023)

53. Origin and arrangement of actin filaments for gliding motility in apicomplexan parasites revealed by cryo-electron tomography

Matthew Martinez*, Shrawan Kumar Mageswaran*, Amandine Guérin, William David Chen, Cameron Parker Thompson, Sabine Chavin, Dominique Soldati-Favre, Boris Striepen, Yi-Wei Chang✉

Nature Communications (2023)

52. Nanoscale details of mitochondrial constriction revealed by cryo-electron tomography

Shrawan Kumar Mageswaran*, Danielle Grotjahn*✉, Xiangrui Zeng, Benjamin Barad, Michaela Medina, My Hanh Hoang, Megan Dobro, Yi-Wei Chang, Min Xu, Wei Yuan Yang, Grant Jensen✉

Biophysical Journal (2023)

51. Inflammasomes primarily restrict cytosolic Salmonella replication within human macrophages

Marisa S Egan, Emily A O'Rourke, Shrawan Kumar Mageswaran, Biao Zuo, Inna Martynyuk, Tabitha Demissie, Emma N Hunter, Antonia R Bass, Yi-Wei Chang, Igor E Brodsky, Sunny Shin✉

eLife (2023)

50. Bdellovibrio predation cycle characterized at nanometre-scale resolution with cryo-electron tomography

Mohammed Kaplan*✉, Yi-Wei Chang*, Catherine Oikonomou, William Nicolas, Andrew Jewett, Stefan Kreida, Przemyslaw Dutka, Lee Rettberg, Stefano Maggi, Grant Jensen✉

Nature Microbiology (2023)

49. High-throughput cryo-ET structural pattern mining by unsupervised deep iterative subtomogram clustering

Xiangrui Zeng, Anson Kahng, Liang Xue, Julia Mahamid, Yi-Wei Chang, Min Xu✉

Proceedings of the National Academy of Sciences (2023)

48. Computational methods toward unbiased pattern mining and structure determination in cryo-electron tomography data

Hannah Hyun-Sook Kim, Mostofa Rafid Uddin, Min Xu✉, Yi-Wei Chang✉

Journal of Molecular Biology (2023)

47. In situ structure determination of bacterial surface nanomachines using cryo-electron tomography

Longsheng Lai, Yee-Wai Cheung, Matthew Martinez, Kathryn Kixmoeller, Leon Palao III, Stefan Steimle, Meng-Chiao Ho, Ben E. Black, Erh-Min Lai, Yi-Wei Chang✉

Methods in Molecular Biology (2023)

46. Structural basis of a transcription pre-initiation complex on a divergent promoter

Jose J. Gorbea Colón*, Leon Palao III*, Shin-Fu Chen, Hee Jong Kim, Laura Snyder, Yi-Wei Chang✉, Kuang-Lei Tsai✉, Kenji Murakami✉

Molecular Cell (2023)

2022

45. An apical membrane complex for triggering rhoptry exocytosis and invasion in Toxoplasma

Daniela Sparvoli*, Jason Delabre*, Diana Marcela Penarete-Vargas†, Shrawan Kumar Mageswaran†, Lev M. Tsypin, Justine Heckendorn, Liam Theveny, Marjorie Maynadier, Marta Mendonça Cova, Laurence Berry-Sterkers, Amandine Guérin, Jean-François Dubremetz, Serge Urbach, Boris Striepen, Aaron P. Turkewitz, Yi-Wei Chang, Maryse Lebrun✉

EMBO Journal (2022)

44. Bacterial outer-membrane polysaccharide export (OPX) proteins occupy three structural classes with selective β-barrel porin requirements for polymer secretion

Fares Saïdi*, Utkarsha Mahanta*, Adyasha Panda, Nicolas Y. Jolivet, Razieh Bitazar, Gavin John, Matthew Martinez, Abdelkader Mellouk, Charles Calmettes, Yi-Wei Chang, Gaurav Sharma✉, Salim T. Islam✉

Microbiology Spectrum (2022)

43. Archaic chaperone-usher pili self-secrete into superelastic zigzag springs

Natalia Pakharukova*, Henri Malmi*, Minna Tuittila, Tobias Dahlberg, Debnath Ghosal, Yi-Wei Chang, Si Lhyam Myint, Sari Paavilainen, Stefan David Knight, Urpo Lamminmäki, Bernt Eric Uhlin, Magnus Andersson, Grant Jensen, Anton Zavialov✉

Nature (2022)

42. Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography

Matthew Martinez, William David Chen, Marta Mendonça Cova, Petra Molnár, Shrawan Kumar Mageswaran, Amandine Guérin, Audrey Ragan Odom John, Maryse Lebrun, Yi-Wei Chang✉

Nature Microbiology (2022)

41. Parasitology meets cryo-electron tomography – exciting prospects await

Liam M. Theveny*, Shrawan Kumar Mageswaran*, William David Chen, Matthew Martinez, Amandine Guérin, Yi-Wei Chang✉

Trends in Parasitology (2022)

40. Discovery of a novel inner membrane-associated bacterial structure related to the flagellar type III secretion system

Mohammed Kaplan, Catherine Oikonomou, Cecily Wood, Georges Chreifi, Debnath Ghosal, Megan Dobro, Qing Yao, Ritesh Ranjan Pal, Amit Baidya, Yuxi Liu, Stefano Maggi, Alasdair McDowall, Sigal Ben-Yehuda, Ilan Rosenshine, Ariane Briegel, Morgan Beeby, Yi-Wei Chang, Carrie Shaffer, Grant Jensen✉

Journal of Bacteriology (2022)

39. Novel transient cytoplasmic rings stabilize assembling bacterial flagellar motors

Mohammed Kaplan, Catherine Oikonomou, Cecily Wood, Georges Chreifi, Poorna Subramanian, Davi Ortega, Yi-Wei Chang, Morgan Beeby, Carrie Shaffer, Grant Jensen✉

EMBO Journal (2022)

2021

38. In situ ultrastructures of two evolutionarily distant apicomplexan rhoptry secretion systems

Shrawan Kumar Mageswaran, Amandine Guérin*, Liam M. Theveny*, William David Chen*, Matthew Martinez, Maryse Lebrun, Boris Striepen, Yi-Wei Chang✉

Nature Communications (2021)

37. The cryo-EM effect: structural biology of neurodegenerative disease aggregates

Benjamin Creekmore, Yi-Wei Chang, Edward Lee✉

Journal of Neuropathology & Experimental Neurology (2021)

36. The cryo-EM effect: structural biology of neurodegenerative disease proteostasis factors

Benjamin Creekmore, Yi-Wei Chang, Edward Lee✉

Journal of Neuropathology & Experimental Neurology (2021)

35. Unsupervised domain alignment based open set structural recognition of macromolecules captured by cryo-electron tomography

Yuchen Zeng, Gregory Howe, Kai Yi, Xiangrui Zeng, Jing Zhang, Yi-Wei Chang, Min Xu✉

Proc Int Conf Image Proc. (2021)

34. An Alveolata secretory machinery adapted to parasite host cell invasion

Eleonora Aquilini*, Marta Mendonça Cova*, Shrawan Kumar Mageswaran, Nicolas Dos Santos Pacheco, Daniela Sparvoli, Diana Marcela Penarete-Vargas, Rania Najm, Arnault Graindorge, Catherine Suarez, Marjorie Maynadier, Laurence Berry-Sterkers, Serge Urbach, Pilar Ruga Fahy, Amandine Guérin, Boris Striepen, Jean-François Dubremetz, Yi-Wei Chang, Aaron Turkewitz, Maryse Lebrun✉

Nature Microbiology (2021)

33. Active learning to classify macromolecular structures in situ for less supervision in cryo-electron tomography

Xuefeng Du, Haohan Wang, Zhenxi Zhu, Xiangrui Zeng, Yi-Wei Chang, Jing Zhang, Eric Xing, Min Xu✉

Bioinformatics (2021)

32. In situ imaging of bacterial membrane projections and associated protein complexes using electron cryo-tomography

Mohammed Kaplan, Georges Chreifi, Lauren Ann Metskas, Janine Liedtke, Cecily Wood, Catherine Oikonomou, William Nicolas, Poorna Subramanian, Lori Zacharoff, Yuhang Wang, Yi-Wei Chang, Morgan Beeby, Megan Dobro, Mark McBride, Ariane Briegel, Carrie Shaffer, Grant Jensen✉

eLife (2021)

31. Loss of the bacterial flagellar motor switch complex upon cell lysis

Mohammed Kaplan, Elitza Tocheva, Ariane Briegel, Megan Dobro, Yi-Wei Chang, Poorna Subramanian, Alasdair McDowall, Morgan Beeby, Grant Jensen✉

mBio (2021)

30. Programmed flagellar ejection in Caulobacter crescentus leaves PL-subcomplexes

Mohammed Kaplan, Yuhang Wang, Georges Chreifi, Lujia Zhang, Yi-Wei Chang, Grant Jensen✉

Journal of Molecular Biology (2021)

2020

29. PilY1 and minor pilins form a complex priming the type IVa pilus in Myxococcus xanthus

Anke Treuner-Lange, Yi-Wei Chang, Timo Glatter, Marco Herfurth, Steffi Lindow, Georges Chreifi, Grant Jensen, Lotte Søgaard-Andersen✉

Nature Communications (2020)

28. Bacterial flagellar motor PL-ring disassembly subcomplexes are widespread and ancient

Mohammed Kaplan, Michael Sweredoski, João Rodrigues, Elitza Tocheva, Yi-Wei Chang, Davi Ortega, Morgan Beeby, Grant Jensen✉

Proceedings of the National Academy of Sciences (2020)

27. Bacteria suit up with virus armor

Yi-Wei Chang✉

Proceedings of the National Academy of Sciences (2020)

2019

26. Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS

Debnath Ghosal*, Kwangcheol Jeong*, Yi-Wei Chang, Jacob Gyore, Lin Teng, Adam Gardner, Joseph Vogel✉, Grant Jensen✉

Nature Microbiology (2019)

25. Simulations suggest a constrictive force is required for Gram-negative bacterial cell division

Lam Nguyen, Catherine Oikonomou, Jane Ding, Mohammed Kaplan, Qing Yao, Yi-Wei Chang, Morgan Beeby, Grant Jensen✉

Nature Communications (2019)

24. De novo structural pattern mining in cellular electron cryotomograms

Min Xu, Jitin Singla, Elitza Tocheva, Yi-Wei Chang, Raymond Stevens, Grant Jensen, Frank Alber✉

Structure (2019)

23. In situ imaging and structure determination of bacterial toxin delivery systems using electron cryotomography

Debnath Ghosal*, Mohammed Kaplan*, Yi-Wei Chang, Grant Jensen✉

Methods in Molecular Biology (2019)

2014

10. Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

Yi-Wei Chang, Songye Chen, Elitza Tocheva, Anke Treuner-Lange, Stephanie Löbach, Lotte Søgaard-Andersen, Grant Jensen✉

Nature Methods (2014)

9. Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling

Ariane Briegel, Mark Ladinsky, Catherine Oikonomou, Christopher Jones, Michael Harris, Daniel Fowler, Yi-Wei Chang, Lynmarie Thompson, Judith Armitage, Grant Jensen✉

eLife (2014)

2013

8. Structural basis of a physical blockage mechanism for the interaction of response regulator PmrA with connector protein PmrD from Klebsiella pneumoniae

Shih-Chi Luo, Yuan-Chao Lou, Mahalingam Rajasekaran, Yi-Wei Chang, Chwan-Deng Hsiao, Chinpan Chen✉

Journal of Biological Chemistry (2013)

2012

7. Crystal Structure of DnaK Protein Complexed with Nucleotide Exchange Factor GrpE in DnaK Chaperone System INSIGHT INTO INTERMOLECULAR COMMUNICATION

Ching-Chung Wu, Vankadari Naveen, Chin-Hsiang Chien, Yi-Wei Chang, Chwan-Deng Hsiao✉

Journal of Biological Chemistry (2012)

6. Interaction surface and topology of Get3-Get4-Get5 protein complex, involved in targeting tail-anchored proteins to endoplasmic reticulum

Yi-Wei Chang*, Tai-Wen Lin*, Yi-Chuan Li, Yu-Shan Huang, Yuh-Ju Sun, Chwan-Deng Hsiao✉

Journal of Biological Chemistry (2012)

2010

5. Structural fold, conservation and Fe (II) binding of the intracellular domain of prokaryote FeoB

Kuo-Wei Hung*, Yi-Wei Chang*, Edward Eng*, Jai-Hui Chen, Yi-Chung Chen, Yuh-Ju Sun, Chwan-Deng Hsiao✉, Gang Dong, Krasimir Spasov, Vinzenz Unger, Tai-huang Huang✉

Journal of Structural Biology (2010)

4. Crystal structure of Get4-Get5 complex and its interactions with Sgt2, Get3, and Ydj1

Yi-Wei Chang*, Yu-Chien Chuang*, Yu-Chi Ho, Ming-Yuan Cheng, Yuh-Ju Sun, Chwan-Deng Hsiao✉, Chung Wang✉

Journal of Biological Chemistry (2010)

2008

3. Crystal structure of the N-terminal domain of Geobacillus kaustophilus HTA426 DnaD protein

Cheng-Yang Huang✉, Yi-Wei Chang, Wei-Ti Chen

Biochemical and Biophysical Research Communications (2008)

2. Crystal structures of the 70-kDa heat shock proteins in domain disjoining conformation

Yi-Wei Chang, Yuh-Ju Sun, Chung Wang, Chwan-Deng Hsiao✉

Journal of Biological Chemistry (2008)

2007

1. Structure of the SARS coronavirus nucleocapsid protein RNA-binding dimerization domain suggests a mechanism for helical packaging of viral RNA

Chun-Yuan Chen, Chung-Ke Chang, Yi-Wei Chang, Shih-Che Sue, Hsin-I Bai, Lilianty Riang, Chwan-Deng Hsiao✉, Tai-Huang Huang✉

Journal of Molecular Biology (2007)